Formulation of copper nanoparticles gel and its characterization by cytotoxicity antimicrobial anti biofilm and time kill assay against periodontal pathogens

Abstract

newline Abstract newlineCopper nanoparticles (CuNPs) have garnered attention in dentistry due to their newlinepotential antimi-crobial properties. The study aimed to assess the minimum newlineinhibitory and bactericidal concentration, cytotoxicity against periodontal and newlinemouse fibroblasts by MTT assay, time-kill assay against periodontal pathogens, newlineand antibiofilm assay by the cell culture method for the prepared CuNPs gel. newlineMinimum inhibitory concentration (MIC) value ranged from 0.4 3.12 µg/mL, newlinewhereas MBC value ranged from 0.4 25 µg/mL for periodontal pathogens. IC50 newlinevalue of Cu-NP against mouse fibroblasts (L939) was 49.12 ± 1.67, and against newlineperiodontal fibroblasts at 3.12%, displaying 98% of cell viability. Time-kill assay newlineshowed no growth of Prevotella intermedia and Tannerella forsythia after 10 min, newlineAggregatibacter actinomycetemcomitans at 30 min, Fusobacterium nucleatum at 4 newlineh, and Porphyromonas gingivalis at 24 h. and weak anti-biofilm activity. Cu-NP newlinegel is cost-effective, eco-friendly, and holds promise for combating infections and newlinepromoting tissue regeneration