Formulation of an efficient blood banking strategy by physical perturbation of red blood cells

Abstract

Erythrocytes are frequently required in clinic and its critical newlinetherapeutic properties are utilized in treating trauma, critically ill and newlinepreoperative patients. Transfused RBCs are expected to deliver oxygen to newlinetissues and cells via blood flow through the circulatory system. Production of newlinean artificial RBC remains myth till to date. Thus, the only and prime source of newlineRBCs is human volunteers. Therefore, the supply of stored RBC in blood newlinebank systems becomes vulnerable to many conditions. Our recent experience newlineof the COVID pandemic situation made the issue worse. As per the guideline, newlineblood from a person, who tested positive and recovered from the disease, can newlinebe collected only after 28 days of the discharge from the hospital or 28 days newlineafter the end of the home isolation. These restrictions exert tremendous newlinepressure on the supply of stored blood and RBCs. Hence, it is necessary to use newlineoptimal storage methods to preserve RBCs that extends RBC life in blood newlinebank system. newlinePreservation and deposition of blood and blood components in a newlineblood bank are always related with the storage lesion , which includes newlinemorphological and metabolic changes, protein damage, and membrane newlinedis-integrity. The interval between the donation and the transfusion is a newlinecritical issue since the time of storage positively correlates with the rate of newlinehemolysis. The maximum accepted shelf life of RBC is 42 days in the US and newline35 days in many European countries. The standard storage buffer used for newlineboth RBC and whole blood is CPDA (Citrate, Phosphate, Dextrose and newlineAdenine) which conserves the blood and its components. newline