Evaluation of blaOXA23 gene in carbapenem resistant Acinetobacter species isolated from various clinical samples

Abstract

Evaluation of blaOXA-23 gene in carbapenem-resistant Acinetobacter newlinespecies isolated from various clinical samples. newline newlineMd. Mustafa Sofiur Rahman newlineDr. Ramanath Karicheri newlineProfessor newlineDepartment of Microbiology newlineIndex Medical College, Indore, M.P. newline(Supervisor) newlineBackground: Acinetobacters are significant nosocomial pathogens. Surgical-site newlineinfections, urinary tract infections, ventilator-associated pneumonia and bacteremia are newlinesome notable infections caused by them, especially in Intensive Care Unit (ICU) newlinepatients. Multi-drug-resistant and pan-drug resistant phenotypes in Acinetobacter newlinespecies are of great concern because they exhibit resistance to several classes of newlineantibiotics thereby limiting the therapeutic options. The worrying trend of carbapenem newlineresistance in A. baumannii owing to the acquisition of a variety of resistance newlinemechanisms is a global concern. In view of the increasing bacterial resistance, regular newlinemonitoring of resistance patterns is necessary to improve guidelines for empirical newlineantibiotic therapy. newlineAIM and OBJECTIVES: To evaluate the blaOXA-23 gene in carbapenem-resistant newlineAcinetobacter spp. isolated from various clinical samples. To Identify and isolate newlineAcinetobacter species from various clinical samples. To detect antibiotic susceptibility newlineprofile of isolated organisms i.e., Acinetobacter species. To detect Carbapenem newlineresistance by isolated Acinetobacter species bacteria and detection of blaOXA-23 gene newlinein isolated organism. newlineMATERIALS AND METHODS: A prospective study was carried out and a total of newline287 samples were collected and included in the study. Bacterial isolation and newlineidentification was done by Grams staining, Cultural characteristics on Blood agar and newlineMacConkey s agar plate. Species were identified by biochemical tests. Antibiotic newlinesusceptibility test was performed by Kirby Bauer Disc diffusion method. newlineCarbapenemase production was screened by mCIM test and confirmed by the newlineModified Hodge test. BlaOXA-23 gene was detected by PCR. newlineResult: The distribution of isolates was, Acinetobacter b