Phytochemical and Pharmacological evaluation of balanites aegyptiaca seeds oil as Anti inflammatory Analgesic and Anti Rheumatic activities

Abstract

Medicinal plants are known to exhibit many biological activities from the ancient newlineperiod. They were applied in the ayurvedic medicines for the treatment of various newlinediseases. Balanites aegyptiaca (L.) del which belongs to the family of newlineBalanitaceae. It is found to be an evergreen xerophytic tree of tremendous newlinemedicinal importance and was found to be distributed throughout the dried parts of newlineIndia. In India and Asia, it was reported to be used in a variety of folk medicines. newlineVarious parts of the plants has been used in the Ayurvedic and other folk newlinemedicines in order to treat different ailments which includes syphilis, jaundice, newlineliver and spleen problems, epilepsy, yellow fever. This present work deals with an newlineattempt to develop a modified novel, simple method for extraction of Balanite newlineaegyptiaca seeds oil for determination of anti-inflammatory, analgesic activity and newlineanti-rheumatic activity. Extraction was done to extract seed oil and the extract newlineobtained was evaluated for phytochemical estimation and result showed presence newlineof alkaloids, glycosides, carbohydrate, steroids, proteins, Saponins, Tannins and newlineflavonoids. The oil obtained after extraction were evaluation for physical newlineparameter showed surface tension of 31mN/m, Viscosity of 24 cp, Acid value of newline1.01mg/g. Along with it extracted oil had a saponification value of 29.5mg/g and newlineIodine value of 59.6mg/g. After obtaining the unsaponified matter the compounds newlinewere isolated by column chromatography using isocratic elution technique. newlineIsolated compounds were analyzed spectrometrically using IR spectroscopy, NMR newlineanalysis and Mass Spectroscopy. In vivo studies conducted to evaluate the toxicity newlinestudy on the extract obtained from Balanites aegyptiaca seed. The results obtained newlinefrom the toxicity studies showed no toxicity on the rats with a dose of 300 newlinemg/kg/day and 600 mg/kg/day. The extracted compound evaluated for the antiinflammatory activity on male wistar rat by carrageen induced paw edema method, newlinethe results obtained from the evaluation showed 51.70% reduced ed