Repetitive DNA Elements Analysis of Ralstonia Solanacearum from Marathwada Region

Abstract

Ralstonia solanacearum is considered as one of the most important bacterial diseases of plants. E.F. Smith firstly described the bacterial wilt in potato, tomato and eggplant in 1896 and subsequently in tobacco in 1908. R. solanacearum is a gram-negative soil-borne bacterium capable of infection of hundreds of vegetable species over more than fifty botanical families, causing bacterial wilt. It deserves special attention, from all other plant pathogenic bacteria, for its high phenotypic and genotypic plasticity, a characteristic that makes disease control extremely difficult. newlineTo investigate the biology, genetic diversity and incidence of this disease in different areas of Maharashtra state and Marathwada region, to provide basis for improved prediction and diagnosis together with formulation of management strategies. R.solanacearum infecting Zingiberaceae and Musaceae family is least documented and is largely unknown. Keeping in view the destructive nature of the diseases and high genetic diversity of R.solanacearum present investigations were carried out. The survey and field investigation was carried out during 2015-2017 in different districts of Marathwada region in Maharashtra, in India. Five host samples were selected for study. Total 37 samples from Banana, 42 samples from Tomato , 35 samples from Turmeric ,34 samples from Brinjal and 39 samples from Chilli, a total of 187 samples were collected from the different districts of Marathwada region, in Maharashtra. newlineFrom these samples 19 isolates from Banana, 17 isolates from Tomato , 14 isolates from Turmeric ,12 isolates from Brinjal and 10 isolates from Chilli, a total of 72 strains were isolated on KZC medium. The colonies were spherical shaped with variable size, smooth margins, and white mucoid colonies with a red center. newline2 newlineBased on 16s ribosomal RNA sequencing the bacterium were identified as Ralstonia solanacearum. The 16S rRNA sequences of the strains were assigned GenBank Accessions numbers KC620463 to KC620472 and MW485843 to MW485852. newlineAmong the seven