Evaluation of neuroprotective activity of carvacrol against neuroinflammation induced by chronic stress in mice

Abstract

newline Long term stressful events in life altering immune system is a major factor for the development of psychopathological conditions By now it s clear that stress induced neuroinflammatory cytokines are the key role in mediating neurochemical and neuroendocrine systems causing the behavioral disorders and cognitive impairment. Current anti-depressants are unable to correct neurotransmitters levels directly or indirectly by inhibiting cytokines expression Present study was conducted to elucidate the effect and mechanism of Carvacrol on chronic restraint stress CRS induced neuroinflammation anxiety like depression, learning and memory impairment in C57BLJ6 male mice Chronic restraint stress was induced for 6 hours/day for 21 consecutive days Before one hour stress induction mice were treated with Carvacrol After stressor cessation behavioural and recognition tests were conducted. At the end of study mice were sacrificed brain and blood samples were collected Cytokines TNF á and IL 6 and neurotransmitters were quantified and AChE activity was determined in prefrontal cortex and estimated corticosterone levels in plasma Histopathological examination of the hippocampus was performed Therefore in our present study, we conclude Carvacrol significantly attenuated behavioral changes with memory defects in chronic stress induced neuroinflammation mice Also our study emphasized the restoration of neurotransmitter levels like NA and 5 HT by reducing cytokines expression CORT levels and protected hippocampal structural change. Therefore Carvacrol could be a potential therapeutic agent for treatment of the Major depressive disorder