Prevalence and risk factors analysis associated with bovine brucellosis and its Public health significance especially in peri urban of brij region of UP

Abstract

During present study an attempt was made to find seroprevalence of newlinebrucellosis in bovine milk, serum, aborted fetal tissue and human serum sample from newlinedifferent peri urban places of four districts of Brij region which include Mathura, newlineAgra, Hathras and Kasganj. A total of 1860 samples comprising of 700 bovine milk newlinesamples, 567 bovine serum sample, 84 aborted fetal tissue sample and 509 human newlineserum sample were processed. Out of 1860 samples, in 700 milk sample species wise newlineprevalence of brucellosis in 450 cattle milk sample was found to be 05.78 % (26/450) newlineand 08.45% (38/450) positive by MRT and I- ELISA respectively. On the other hand newlinein buffaloes milk the prevalence of brucellosis in 250 milk sample was found to be newline07.20% (18/250) and 09.02% (23/250) positive by MRT and I- ELISA respectively. newlineIn 567 animal serum sample the prevalence of brucellosis in 391 cattle was found to newlinebe 07.93% (31/391), 08.69% (34/391) and 10.74% (42/391) shows positive by RBPT, newlineSTAT and I- ELISA respectively and in 176 buffaloes tested serum sample the newlineseroprevalence was found to be 09.66% (17/176), 10.79% (19/176) and 12.5% newline(22/176) positive by RBPT, STAT and I- ELISA respectively. Out of 509 tested newlinehuman serum sample (male 394 and female 115), the seroprevalence of human newlinebrucellosis was found to be 02.75% (14/509), 04.31% (21/509) and 03.73% (19/509) newlineshows positive by RBPT, STAT and I- ELISA respectively. A total of 228 samples, newline144 ELISA positive samples (61bovine milk, 64 bovine serum and 19 human serum newlinesamples) and 84 tissue samples were screened by PCR to detect the presence of newlineBrucella genus specific gene (bcsp31, omp2 and 16S rRNA) and Brucella abortus newlinespecies specific IS711genes. A total 49 samples were found positive for Brucella newlinegenus specific genes. Seroprevalence of brucellosis in serum, milk and aborted fetal newlinetissue depicts a dangerous picture regarding human safety because milk is essential newlineand complete food for human beings. So proper precautions to be taken while newlinehandling brucellosis infected ani