Pharmacognostical And Phytochemical Investigation Of Bryonia Laciniosa

Abstract

Bryonia laciniosa Linn. (F: Cucurbitaceae) is commonly known as Shivlingi, a newlinewell-known Ayurvedic and Homeopathic medicine used in the treatment of newlineinfertility, inflammation and impotency. There are elfin reports on the study of this newlineplant. Thus, the present work was aimed at pharmacognostical and phytochemical newlinestudy of B. laciniosa. The pharmacognostical study revealed the important newlineidentifying characters of the plant. The plant is annual herb with bifid tendrils in the newlineaxils of the leaf, consisting striped fleshy fruit and shivlingi shaped seed. The newlinemicroscopical study showed that the leaf is dorsiventral with anomocytic stomata. newlineThe old stem and old root were characterized by secondary vascular growth, fruit newlineconsists of rich endosperm and seeds have characteristic pigment layer. newlinePhysicochemical study of different parts of plant was performed and it revealed that newlineleaves consist of more foreign organic matter, total ash value and extractive value newlinecompared to other parts. The phytochemical screening showed the presence of newlinecarbohydrates, proteins and amino acids, sterols, saponins, flavonoids, alkaloids, newlinephenols and tannins. newlineTotal Phenolic Content and Total Flavonoid Content were determined in stem, newlineleaves, fruit, seeds and root of B. laciniosa by Folin Ciocalteu and Aluminium newlinechloride method respectively. The results showed that leaves contain maximum newlineTotal Phenols and Total Flavonoids and root contains the minimum. The leaves newlinefound to contain 9.2 % of Total Phenols and 4.48 % Total Flavonoid. newlineTLC study of flavonoid rich ethyl acetate extract using Ethyl acetate: Formic acid: newlineGlacial acetic acid: Water (100: 11: 11: 26) as solvent system, showed the presence newlineof two prominent spots with Rf value of 0.55 and 0.91. These two were isolated by newlinepreparative TLC using same solvent system and were identified and characterised newlineby TLC, UV, FTIR, 1HNMR and HRLCMS studies. Based on the TLC and spectral newlinestudies, the compounds were identified as isoquercetin and quercetin. newlineTLC of sterol containing pet