Studies on molecular characterization and tissue specific expression profile of alpha1 sodium potassium adenosine triphosphatase ATP1A1 gene in riverine buffaloes bubalus bubalis

Abstract

The present study enriches the bubaline genomic data as it has generated sequence information of the ATP1A1 gene in riverine buffaloes. Further, the study has helped to compare the sequences of selected exonic regions of the buffalo ATP1A1 gene with several Indian native cattle breeds. The identified variations identified in the ATP1A1 gene could be evaluated in future for their role in heat tolerance traits in riverine buffaloes and cattle, as this gene is known to play a significant role in heat stress response. The present study also helped in the identification of a panel of stably expressed housekeeping genes that could be utilized for functional/transcriptional studies in riverine buffaloes. The expression pattern of all alpha isoforms of the Na+/K+-ATPase gene across various tissues of buffaloes indicated their tissue-specific expression characteristics. This study has also helped to understand the heat-responsive role of ATPase alpha isoforms. In future, such an effort will pave the way to study the plausible functional role of Na+/K+-ATPase isoforms and their variants in regulating heat tolerance traits in riverine buffaloes and native cattle. newline