Quantitative Evaluation of Some Important Phytoconstituents in Polyherbal Formulation

Abstract

Phytomedicines are flooding the markets of developed countries, and consumers all over the world prefer natural-based formulations. If the principal active component is known, it is most logical to quantify this compound. To achieve the desired therapeutic effect, an optimal concentration of therapeutically active components is required; this can be accomplished through the application of various modern tools and techniques which analyse these compounds in a mixture. So, present investigation was undertaken with a view to evaluate polyherbal formulations for quantitative estimation of therapeutically active phytoconstituents which can prove ethno-medicinal value of the polyherbal formulation. A RP-HPLC technique was developed and validated for quantification of Ascorbic acid, Gallic acid, Quercetin, and newlineReserpine from the methanolic extract of formulation (Cardostab tablet) and crude newlinepharmaceuticals, using PDA as a detector and Gradient elution mode for the mobile phase. newlineGradient mode was used to separate the samples on an Agilent eclipse C18 column (25 cm, 4.6 mm, 5 m). Phosphate buffer pH 4 and Acetonitrile make up Mobile Phase A and B, respectively. At 227 nm UV detection, the flow rate was 1 mL/min. In the range of 50-250 and#61549;g/mL for Ascorbic acid, 100-300 and#61549;g/mL for Gallic acid, 100-300 and#61549;g/mL for Quercetin, and 50-250 and#61549;g/mL for Reserpine, the method was linear. Precision was less than 2% at both the interday and intraday levels, with accuracy ranging from 98 to 102 percent. For the measurement of Ascorbic acid, Gallic acid, Quercetin, and Reserpine, an HPTLC method was devised and validated. The newlineisolation was carried out on the CAMAG HPTLC system with the Linomat V sample applicator newlineand the winCATS Planar Chromatography Manager software. The stationary phase was TLC silica gel 60F254, while the mobile phase was Toluene: Ethyl acetate: Methanol: Glacial acetic acid (7: 2: 1: 0.3 v/v) and detected at 254 nm using a D2 lamp.