Hepatoprotective and Antioxidant Properties of Abutilon Indicum A Comprehensive Study on Phytochemical Analysis and Therapeutic Impact Against Isoniazid and Rifampicin Induced Hepatotoxicity and Proinflammatory Marker Gene Expression in Wistar Rats

Abstract

This paper focuses on the hepatoprotective and antioxidant effect of Abutilon indicum, a medical plant used in the treatment of liver diseases, against Isoniazid and Rifampicin induced hepatotoxicity in Wistar rats. The present study was designed to assess the potential of Ethanolic Leaf Extract of Abutilon indicum in hepato toxicity caused by INH and RIF. A preliminary phytochemical study of ELEAI was done to determine the various bioactive compound present in this plant, this was done both qualitatively and quantitatively. In Vitro studies of INH and RIF induced hepatotoxicity and in Vivo studies of INH and RIF induced hepatotoxicity were done using Wistar rats. Phytochemical research further established the presence of Flavanoids in ELEAI some of which are Luteolin. The DPPH experiment revealed substantial antioxidant activity comparable to Ascorbic acid. While ELEAI treatment increased antioxidant enzyme activity it also markedly decreased liver enzyme levels and oxidative stress indicators MDA.proinflammatory marker gene expression, TNF alpha and NF kappa B was dose dependently regulated by ELEAI. Histological examination verified that the ELEAI treated groups had less inflammation and liver damage.These findings support the traditional usage of Abutilon indicum in liver disorders and demonstrate its potential as a natural alternative for liver disease management. Further research is required to understand its underlying mechanisms and clinical utility.