Investigations on toxicological Behavior of nano sio2 al2o3 tio2 and Zro2 particles in different Environments

Abstract

Nanoparticles have wide applications in many fields from newlineelectronics to biomedicine Their immense use may cause metal accumulation newlinein the ecosystem as well as in food chain resulting in metal toxicity Thus the newlinepresent study aimed at investigating the toxicity of nano scale SiO2 Al2O3 newlineZrO2 and TiO2 on different biological systems from bacteria to animal cell newlineline and comparing their respective bulk micro level counterparts newlineMicro particles of analytical reagent AR grade are obtained from newlineLoba Chemie and Merck India SiO2 Al2O3 TiO2 and ZrO2 nanoparticles newlineare synthesised from natural sources biomass rice husk bauxite illuminate newlineand zircon sand employing different methods and are characterised using newlinedifferent techniques Effect of the metal oxides on selected PGPR newline Pseudomonas fluorescens Bacillus megaterium Bacillus brevis and newlineAzotobacter vinelandii is analysed by performing susceptibility studies in newlinesaline and water medium Disc diffusion method is employed to reveal their newlinetoxicity towards PGPR In addition their effect on soil nutrient is studied newlineusing the standard estimation protocol 3 4 5 dimethylthiazol 2 yl 2 5 newlinediphenyl tetrazolium bromide reagent MTT assay on murine embryonic newlinefibroblasts cell line NIH 3T3 and di phenyl 2 4 6 trinitrophenyl newlineiminoazanium DPPH assay are performed to check the in vitro cytotoxicity newlineand antioxidant potential of the selected metal oxides Effect of metal oxides newlineon the growth chlorophyll and protein contents of Porphyridium aerugineum newlineis studied based on the standard analysis protocols newline newline