Phenotypic and genotypic characterisation of trichosporon species from clinical samples

Abstract

Trichosporon sp have been recognized as an emerging yeast infection which do not respond to the routinely used antifungal agents used to treat yeast infections A total 72 clinical isolates were considered for the study The phenotypic characteristion identified our isolates as 64 72 T asahii, 5 72 T asteroides 2 72 T cutaneum and 1 72 T mucoides Genotypic characterisation techniques finally identified the clinical isolates as 65 90 point 3 percent T asahii 4 5 point 5 per cent T cutaneum 2 2 point 8 per cent T loubieri and one as Trichosporon sp closely related to T ovoides Fingerprinting by RAPD primer GAC1 identified more heterogeneity among the T asahii isolates than M13 Amphotericin B fluconazole and itraconazole had high GM MICs MIC50 and MIC90 values indicating that they may not be the right choice to treat infections caused by Trichosporon sp Voriconazole posaconaole and ravuconazole are the most potent antifungal agents in treating Trichosporon infections since they had low GM MICs MIC50 and MIC90 values for both T asahii and non T asahii sp Trichosporon biofilms were intrinsically resistant to all the antifungal agents tested Hence correct identification of the Trichosporon species along with their antifungal susceptibility pattern helps the clinician in providing appropriate treatment to the patient newline newline newline