Formulation characterization and evaluation of Aquasomal dry powder inhaler of antiviral peptide

Abstract

The research aims to enhance understanding of aquasome preparation, offering insights into newlineoptimizing formulations for potential applications in drug delivery systems. Antiviral peptide containing aquasomes composed of lactose and trehalose as oligosaccharides encapsulate calcium newlinephosphate cores. The cores synthesized by sonication and co-precipitation methods were screened based on % yield for calcium phosphate synthesis. Various coating methods were employed for the screening based on carbohydrate loading capacity. The study evaluates these formulations to optimize carbohydrate loading and particle size for potential applications in bioactive drug newlinedelivery to enhance drug loading capacity and minimize use of bioactive drugs for initial newlinescreening and optimization. Ceramic core preparation via sonication yielded higher percentages (69.6%) compared to co-precipitation (54.06%). Trehalose demonstrated superior carbohydrate newlineloading (67.74%) compared to lactose (55.81%) using the lyophilization method. The PlackettBurman design identified newlinefour significant factors affecting particle newlinesize, which are sonication time, amplitude, newlinetemperature, and carbohydrate concentration. The Central Composite Design (CCD) optimization revealed that increased sonication time and carbohydrate amount led to higher carbohydrate loading. Lower sonication temperatures resulted in greater carbohydrate loading. Particle size decreased with increased sonication time but increased with higher carbohydrate amounts and sonication temperatures. The optimized formulation achieved a carbohydrate loading of 104.17 ± 0.021 mg and a particle size of 407.2 ± 0.34 nm, closely matching predicted newlinevalues. The study shows sonication time, temperature, and carbohydrate concentration influence aquasome development. Essential for optimizing their performance in various biomedical applications, particularly in drug delivery.