Chemotyping chemical fingerprinting and artificial neural network modeling in medicinally important Justicia adhatoda and Bacopa monnieri

Abstract

Justicia adhatoda L. (Acanthaceae) is a perennial large dicotyledonous shrub used for newlinetreating cold, cough, bronchitis and respiratory illness and another important plant is newlineBacopa monnieri L. (Scrophulariaceae) known for its immense medicinal value newlinemainly for the treatments of neurological ailments is a small creeping herb. The newlinepresent study deals with chemotyping, chemical fingerprinting and artificial neural newlinenetwork modelling in medicinally important Justicia adhatoda and Bacopa monnieri. newlineIn this study using an efficient HPLC and HPTLC technique the quantitative analysis newlineof vasicine and vasicinone compounds in 120 accessions of Justicia adhatoda and newlineluteonin, apigenin, bacopaside I, bacoside A3, bacopaside II, jujubogenin isomer of newlinebacopasaponin C, bacopasaponin C and bacopaside V in 81 accessions of Bacopa newlinemonnieri has been performed. The analysis result of J. adhatoda leaf sample revealed newlinethat vasicine and vasicinone content varied from 0.01-1.31% and 0.00-0.18% newlinerespectively. Similarly, in B. monnieri eight compounds showed remarkable variation newlinei.e. 0.00-0.05% (luteolin), 0.01-0.43% (apigenin), 0.00-0.53% (bacopaside I), 0.06- newline0.71% (bacoside A3), 0.05-1.53% (bacopaside II), 0.02-0.88% (Jujubogenin isomer of newlinebacopasaponin C), 0.04-0.94% (bacopasaponin C) and 0.00-0.07% (bacopaside V). It newlinehas revealed the presence of two different types of chemotype viz. one is newlinebacopasaponin C rich and bacopaside II rich in Odisha and West Bengal respectively. newlineThe development of HPLC and HPTLC representative chromatographic fingerprint newlineperformed by selecting 24 chromatograms out of 81 batches of B. monnieri samples newlineand 120 batches of J. adhatoda samples from different location of Odisha and West newlineBengal provinces of eastern India. The established chromatographic fingerprint would newlinebe useful for quality control study. Moreover, an ANN with MLP based yield newlineprediction model namely 13-11-1 and 12-06-1 architecture for bacoside A in B. newlinemonnieri and vasicine in J. adhatoda respectively have been developed to check the newlineinfluence of ec