Molecular Characterisation of Helicobacter Pylori from Gastric Biopsy Specimens in Acid Peptic Disease

Abstract

newline Molecular characterisation of Helicobacter pylori from gastric biopsy specimens in newlineacid peptic disease newlineBackground: Helicobacter pylori (H pylori) is a fastidious, microaerophilic bacterium newlinepresent in the human gastric mucosa of 70-90% of the population in developing newlinecountries. It plays a significant role in the pathogenesis of chronic gastritis and peptic newlineulcer disease. Inspite of available diagnostic methods to detect H pylori there is no newlinesingle technique that can meet the criteria in identification of the bacterium. The cagA, newlinewhich is not present in every H pylori strain is a marker for pathogenicity island and is newlineassociated with more severe clinical outcomes. However there are very few studies newlinelooking directly at the presence of cagA gene in gastric antral biopsies of patients. newlineTherefore the present study was done to evaluate PCR for detection of H pylori cagA newlinegene in gastric biopsies as well as their corresponding H pylori isolates, to evaluate newlinediagnostic tests for identification of H pylori and to assess their antimicrobial newlinesusceptibility pattern. newlineObjectives: 1. To study the relative proportion of cagA virulence marker in newlineHelicobacter pylori isolates and gastric biopsy samples by polymerase chain reaction. newline2. To evaluate the sensitivity of culture technique for detection of Helicobacter pylori newlinefrom gastric biopsy samples. newline3. To generate baseline data on antibiotic sensitivity pattern of H pylori. newlineMethodology: Biopsies of gastric tissue from 200 patients with disorders of the upper newlinegastrointestinal tract were studied for H pylori detection by various methods like rapid newlineurease test, culture, Gram s stain, Giemsa stain, Haematoxylin and Eosin stain, newlineantibiotic susceptibility testing of the isolated strains, PCR for 16S rRNA and cagA newlinegene. newlineXIX newline newlineResults: Out of 200 patients H pylori was detected by rapid urease test in 26.5%, newlineGram s staining in 18.5%, Giemsa staining in 29.5%, HandE staining in 14.5%, culture newlinein 2.5%, PCR for 16SrRNA in 48.5% and PCR for cagA in 31%. H