Phytochemical and Pharmacological evaluation on Kigelia africana and Rotula aquatica lour

Abstract

Recent literatures widely acknowledge the role of oxidative stress in the pathophysiology of different disorders like hepatotoxicity, nephrotoxicity, diabetes, cardiovascular diseases etc. Diabetes mellitus especially type 2 diabetes mellitus became pandemic throughout the globe. Helminth infections are generally less or not fatal, they are connected with high rates of morbidity, with chronic infection frequently leading to anemia and malnourishment. newline Diabetes mellitus is a major heterogeneous endocrine and metabolic disorder, characterized by altered metabolism of carbohydrate, lipid and protein, which leads to hyperglycemia and causes many physiological complications, such as hyperlipidemia, hypertension and atherosclerosis. The global prevalence of diabetes among adults as per WHO, was estimated at 150 million in 1995, and this is projected to increase to 300 million by 2025. The oxidative stress, caused by free radicals induced by hyperglycemia, contributes to the development and progression of diabetes along with secondary complications. The plants containing natural antioxidants (tannins, flavonoids, vitamin C and E, etc.) that can preserve and#946;-cell function and prevent diabetes induced ROS formation and many plant species are known in folk medicine of different cultures to be used for their hypoglycemic properties and therefore used for treatment of DM. newline In vitro antioxidant activity of methanol and aqueous extract of the Kigelia africana and Rotula aquatica lour leaves and its phenolic and flavonoid content were investigated using different assay method. Methanolic extract of both plants showed concentration dependent and higher significant antioxidant activity. DPPH (2, 2-diphenyl-1-picryl hydrazyl) and reducing power assay (RPA) were carried out to evaluate in vitro antioxidant activity. Total phenolic content was estimated by using Folin-Ciocalteu s reagent and total flavonoid content by Aluminium chloride colorimetric method. newline Anthelmintic activity of methanolic extract of the Kigelia afr