Investigation of Targeted Anti Inflammatory And Hepatoprotective Potential of Novel Standardized Enriched Extracts of Anaphalis Tripenervis Linn

Abstract

The continuous practice of traditional medicines provides scientific validation of the use of herbal medicines, which eventually leads to widespread recognition among the socioeconomic healthcare systems. A significant problem with substantial morbidity and mortality of liver injury is associated with systemic inflammatory reactions. In humans, single nucleotide toll-like receptor (TLR4) gene polymorphisms affect the transduction of the signal and the associated risks of specific diseases, including cirrhosis of the liver and hepatitis, etc. Liver injury in the form of fibrogenic cells in the hepatic stellate cells (HSCs) that interplay with TLR4 further interaction may responsible for inflammation, phenotype, fibrogenesis, and anti-apoptotic properties of hepatocytes. The regulation of injury simultaneously by TLR4 and nuclear factor erythroid 2- related factor (Nrf2) pathways is usually recognized. In this research, the primary focus was on investigation of the hepatoprotective effect of Anaphalis triplinervis extracted with hydroethanolic solvent (HEEAT) against carbon tetrachloride-induced hepatic injury in rats and elucidate the inflammatory cascade leading to apoptosis in liver. Adult Wistar rats were randomized into 6 groups and each group contains six animals, (n=6). Group I: normal control; animals treated with vehicle. Group II: toxin control; rats administered with CCl4+Olive oil 1:1 i.p., Group III: standard; silymarin (125mg/kg), Group IV, V, and VI administered with HEEAT 100, 200, 300 mg/kg up to 14 days. The body weight and haematological assessment were performed and it was found that in the Group VI the hemoglobin, RBC, platelet content was significant 14.81gm/dl, 10.03µl, 12.58µl as compare to the Group II. Further the antioxidant activity was determined by assay for catalase, glutathione reductase, superoxide dismutase, and malondialdehyde in the hepatic tissue. The extract s yield was discovered to be 12.31 percent.