The influence of codon content and mrna secondary structure on heterologous protein expression in ecoli

Abstract

The heterologous protein expression has brought radical changes in newlinerecombinant protein production The large scale production of therapeutic newlinediagnostic and industrially important proteins or enzymes greatly depend on newlinethe protein expression strategies The prime objective of heterologous protein newlineexpression is to obtain higher yield of target proteins But not all proteins are newlinesuccessfully expressed in Ecol Many factors such as vector and host newlinestrength of promoters inducers concentration media composition toxicity etc newlineare known to have influence in protein expression newline