Bioprocess Intensification Strategy for Glucose Oxidase and Surfactin Production Towards Multifaceted Biotechnological Applications

Abstract

This study embarks on the transformative investigation of utilizing Casuarina newlineequisetifolia biomass as a renewable feedstock for the production of high-value newlinebioproducts through an integrative bioprocessing framework. The bioconversion process newlineinitiates with the pretreatment of C. equisetifolia biomass to liberate fermentable sugars, newlineserving as foundational intermediates for downstream biochemical synthesis. Among the newlinepretreatment methodologies assessed, alkaline pretreatment demonstrated superior newlineefficacy, yielding 450 mg of reducing sugars per gram of biomass within 30 minutes at newline120°C. This approach optimizes sugar recovery as well as achieves economic viability, newlinewith processing costs as low as 0.83 USD per kilogram. newlineThe resultant sugar-rich hydrolysate was subsequently subjected to catalytic newlineconversion using silica microspheres, yielding 21.9% hydroxymethylfurfural (HMF), a newlineversatile platform chemical with applications in polymer science and fine chemical newlinesynthesis. Concurrently, microbial fermentation of the hydrolysate facilitated the newlinebiosynthesis of gluconic acid, achieving a yield of 27.8 mg per gram of biomass under newlineoptimized conditions of pH 4.0 and 40°C. Gluconic acid, recognized for its chelating newlinecapabilities and industrial applicability, underscores the multifunctionality of this newlinebioprocessing pipeline newline