Protein engineering of 946 1 4 endoglucanase and chimera construction with 946 glucosidase from clostridium thermocellum for improving ligno cellulosic biomass saccharification

Abstract

Plant cell wall were composed of polymers for example cellulose various hemicellulose and lignin and can be converted into alcohol that can be served as renewable source of energy Saccharification of cellulose major component of plant cell wall requires three major enzymes i e endoglucanases and exoglucanases cellobiohydolases and 206 178 glucosidase for its complete hydrolysis But the use of these enzymes are limited because of their low catalytic efficiency and individual production of each...