Development of biomimetic photo responsive artificial carriers for the transmembrane chloride transport

Abstract

Thesis Abstract Title Development of biomimetic photo responsive artificial carriers for the transmembranechloride transportThe transport of ions across the cell membrane through naturally occurring ion carriers and ionchannels is crucial for multiple physiological functions In particular the transport of chlorideions is known to induce chloride mediated apoptosis inside the cancer cells Traditionaltransporters lack ion selectivity and hence can damage the normal healthy tissues Stimuli suchas voltage pH enzymes ligands light etc have been employed for activating ion transportersinside the cancer cells Light responsive ion transport systems in particular are importantsystems because of their spatiotemporal control remote addressability and less cytotoxicity Herein we developed several light responsive synthetic ion carriers which weremanifestly controlled utilizing the external electromagnetic radiations Initially the mostwidely used azobenzene photoswitch was used to generate the light responsive ion transportsystems and efficient 8220 OFF ON 8221 photoreguatory ion transport activity was achieved Acylhydrazone based photoswitches were employed to enhance the exciting state thermalstability The reversibly gated ion transport activity was achieved utilizing light and catalyticacid respectively as the external stimuli Phenylhydrazone based photoswitches were utilizedto enhance the excitation wavelength These photoswitches function comparatively at higherwavelengths compared to that of acylhydrazone switches Eventually we demonstrated an onitrobenzyl based photocaged ion transport system could be selectively activated inside thecancer cells to induce chloride mediated apoptosis utilizing the external electromagneticradiations newline newline