Genetic Variability and Mapping of Nutritional and Oil Quality Traits in Groundnut Arachis hypogaea L

Abstract

The present investigation was carried out to elucidate the information on genetic newlinevariability and correlation analysis for yield, yield components, nutritional and oil quality traits, newlinediversity with respect to nutritional and oil quality parameters, stability of genotypes for newlinenutritional and oil quality traits and marker identification for nutritional and oil quality newlineparameters in groundnut. The experimental material consisted of 189 accessions of minicore newlinecollection, few advanced breeding lines, controls and 268 RILs (TAG24xGPBD4). They were newlineevaluated in two distinct seasons (kharif 2008 and summer 2008-09) and at two locations newline(UAS Dharwad and ICRISAT Hyderbad). There was significant variation among the newlinegenotypes of minicore collection and RILs population for yield, yield components, nutritional newlineand oil quality traits. newlineThe PCV and GCV indicated the presence of wide genotypic and phenotypic variation newlinefor all the traits studied. Correlations between protein and oil content, oil content and oleic newlineacid, O/L ratio were negative. Oleic acid had negative correlation with linoleic acid. Genetic newlinedivergence study revealed that oleic acid and protein content contributed more (59.53%) newlinetowards genetic diversity. Stability analysis indicated significant G × E interaction for newlinenutritional and oil quality traits. This germplasm viz., ICG2381, ICG6913, ICG5286, ICG6766, newlineICG10185 (gt65%) and RILs No.viz.,187, 191, 193, 234, 252 (gt50%) found stable and had newlinehigh oleic acid content compared to check GPBD4 (50%).Single marker analysis with CAPS, newlineconfirmed their potentiality to identify one mutant allele ahFAD2A of oleoyl-PC desaturase newlineand found to associated with high oleic trait. Linkage analysis using 68 polymorphic markers newlinein RILs population been able to map only 57 markers on 14 linkage group spanning 453.6 cM newlinewith average distance of 7.69 cM. The marker ahFAD2A (8.60-31.64% phenotypic variance) newlineand TCIA01 (5.85-20.84% phenotypic variance) and QTL flanked by markers ahFAD2A newlineTC3H02 (4.95-21.42% phenotypic variance) were found to b