Molecular and genetics basis of amylase resistant starch variation in bread wheat Triticum aestivum L

Abstract

The modern lifestyle and nutritionally poor food supply have raised the concern of obesity and health related issues. To address the later, this study aimed to enhance nutritional quality of bread wheat by altering amylose content. In order to generate variation for grain amylose/Resistant starch content in Indian wheat genotypes a mutant population was developed in background of C 306 . The mutant population had a variation of amylose content (3% to 75%). Two lines TAC 75 and TAC 6 having amylose content 64% and 7% respectively was used to study the molecular basis of trait. The gene expression analysis of starch pathway genes in these lines indicated the role of GBSSI and SBEII in mutants. The global transcriptome analyses further identified a number of differentially expressed genes that provide the candidate to study the regulation of starch biosynthesis. The high amylose trait was transferred to an Indian wheat variety, WH 1105 (high yielding variety) from TAC 75 . The advanced F2 and F3 populations were sequenced using GBS technology and QTL mapping was performed using 1,572 SNP markers. The QTL mapping identified 12 major effect QTLs. Identification of the QTL genes under the two stable QTLs identified 265 protein coding genes, including two genes GBSSI and Sucrose synthase SUS1 as the potential candidates. newlineAltogether, the mutant population, DEGs, and QTLs will facilitate the breeding and MAS for amylose content in Indian genotypes. The lines with high resistant starch content are good preventive measure for the diabetes and related health issues. newline newline