Phytochemical and Pharmacological Evaluation of Medicinal Plants on Type II Diabetic and Its Complication

Abstract

The plant Clerodendrum viscosum was selected for the study based on the literature review. From the plethora of the literature we found that the plant has been used widely for various ailments of mankind. Still the plant has not been validated for the phytochemical and pharmacological activity. The plants were collected from the Palghat district in Kerala. The plant were identified by the botanical survey of India, Coimbatore the Ref No. IS BSI/SRC/5/23/10-11/Tech1152. The roots of the plant were dried and powdered. Various qualitative studies like ash value, extractive value, florescent analysis where done and the powdered drug was extracted according to the polarity of the solvent. The extracts were subjected to preliminary phytochemical analysis and found for the presence of sterols, flavonoids, phenolic compounds and glycosides. The total flavonoid content was estimated in each extract using Gallic acid as a standard and found that the ethyl acetate extract haves high concentration of phenolic content. So the study was focused on the ethyl acetate extract. Presence of flavonoids in the extract was confirmed by HPTLC study. The flavonoid rich ethyl acetate extract was subjected to column chromatography and nine fractions were obtained. The fractions obtained from the column chromatography study were subjected to HPTLC finger print analysis. The fractions which shows single band in the HPTLC finger print analysis were taken for HPLC study. This research outcome may be used to lower the impact of diabetes particularly in rural population. Many effective drugs may be compounded based on herbal formulations after accepted scientific step-wise documentations and clearance from authorities involved in the process and in the process. Flavonoids in Clerodendrum viscosum roots may have a promising role as an anti-diabetic. However, with the expansion of knowledge in holistic medicine one may presume the modern day revolutionism may find a compromise in health care and in rationality to provide treatment to the sufferers.