Effect of different centrifugal speed on sperm motility and dna integrity

Abstract

ABSTRACT newlineHuman ejaculate is composed of a mix of spermatozoids, seminal liquid, epithelial cells, immature and newlinenecrotic sperm cells; red and white blood cells and bacteria. The semen sample consists of suspension of newlinespermatozoa stored in the epididymis. At ejaculation, it is mixed with the secretions of the accessory newlineglands. These glands are mainly the prostate and the seminal vesicles, while the bulbourethral glands and newlinethe epididymis represent only the minor contribution of the ejaculate. The two main fractions are present newlinein the seminal fluid; the firstone is prostatic (rich in spermatozoa) and the last fraction of the semen newlineconsists of vesicular fraction (less rich in spermatozoa). During ejaculation, it is very important to collect newlinethe entire volume of the sample: if the first fraction (rich in spermatozoa) is lost, the assessment of the newlinesemen features will be more difficult (Natali, 2011). Under in vivo conditions, spermatozoa are separated newlinefrom these detrimental components in the female genital tract by migration through the cervical mucus. newlineSeveral semen separation techniques have been developed to separate the sperm 4 fraction for use in newlineassisted reproductive techniques. The most commonly used protocols are density gradient centrifugation newline(DGC) and swim-up (SUP). The SUP technique relies on the ability of the motile spermatozoa to swim up newlineinto the culture medium, while slow and immotile sperm remain behind, along with other components in newlinethe semen pellet. The DGC method separates spermatozoa according to their density and favors the newlineisolation of motile and morphologically normal spermatozoa newlineAIM: The aim of the study is to compare the post wash motility and DNA fragmentation levels after newlineprocessed at various centrifugal speed. newline