Coloning expression production purification and characterization of novel glutaminase free recombinat l asparaginase ii of pectobacterium carotovorum mtcc 1428 and erwinia carotovora subsp atroseptica scri 1043 in escherichia coli

Abstract

Bacterial L asparaginase is used as a therapeutic agent for the treatment of acute lymphoblastic leukemia and a processing aid in the manufacture of starchy food products such as potato chips and biscuits to reduce the formation of acrylamide L asparaginase is also used as a model enzyme for the development of new drug delivery system and L asparagine biosensor to diagnose leukemia The different side effects of this drug are attributed due to the presence of partial glutaminase activity of L