Identification Isolation and Anti Inflammatory assessment of phytocompounds from selected North Eastern medicinal plants An Invitro Approach

Abstract

Inflammation is a vital biological response to injury and infection playing a key role in numerous pathological conditions With the growing incidence of chronic inflammatory diseases and the limitations of conventional therapies plant based medicines have emerged as promising alternatives due to their diverse pharmacological properties and reduced side effects This study focuses on the pharmacognostical and phytochemical evaluation of three underexplored plants Zanthoxylum armatum seeds Litsea salicifolia leaf and bark and Mesua assamica fruit Comprehensive phytochemical profiling revealed the presence of significant secondary metabolites including phenolic compounds flavonoids coumarins and tannins Antioxidant assays such as DPPH ABTS and TBARS were employed to determine the most effective extract identifying the ethanol extract of Litsea salicifolia bark as the most potent This extract was subsequently subjected to activity guided fractionation leading to the isolation of two active compounds C1 and C2 Anti inflammatory assays conducted on THP1 cells demonstrated that C2 exhibited superior anti inflammatory activity compared to C1 Further investigations involved insilico studies to predict the binding affinity of C2 to inflammation related targets alongside Western blot analyses to elucidate the molecular mechanisms through the NF and#954;B signaling pathway These findings underscore the anti inflammatory potential of these less studied plants and contribute to the development of novel plant derived therapeutic agents for managing inflammatory conditions newline