Comparative study ofnano elicitors and their role on growth and development of Broccoli Brassica oleracea var italica under in vitro conditions

Abstract

The present study Comparative study of nano elicitors and their role on growth and development of broccoli (Brassica oleracea var. italica) under in-vitro conditions was carried out at Plant Tissue Culture Laboratory, Division of Plant Physiology, Faculty of Basic Sciences, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu during 2020 to 2023 to develop efficient sterilization, callusing, and regeneration protocol under in-vitro response using various explants of broccoli. The effect of nanoparticles (40nm) alone or along with other PGRs was studied. There were six treatments viz. Zinc oxide, Copper oxide, silver nitrate, Chitosan, Salicylic acid, and Methyl jasmonate with three different concentrations 10 mg/l,20 mg/l, and 30 mg/l.The result showed that the maximum surface sterilization of leaf explants of Broccoli (Brassica oleracea var. italica) was achieved by treatment comprising of 1 % (w/v) bavistin for 5 minutes duration followed by 0.50% (v/v) sodium hypochlorite for 3 minutes and mercuric chloride 0.1% for 0.30 minute which gave 88.75% uncontaminated cultures and was highest as compared to all other treatments. Maximum callus induction frequency(CIF) (90.47%), was observed on MS media supplemented with 1.5 mg/l 2,4-D and 0.75 mg/l, Chitosan showed the maximum callus induction frequency (CIF)(90.27%), followed by 87.45% CIF on culture media supplemented with 1.0 mg/l 2,4-D + 0.75 mg/l chitosan. On the other hand, no response was seen on media without growth regulators and nanoparticles. The combination of half-strength MS basal medium, along with a specific mix of 2.0 mg/l NAA and 0.5 mg/l IBA, was most effective in improving root growth.This exceptional medium produced an impressive 84.25% rooting rate, with roots growing to a remarkable length of 4.50 cm.Maximum shoot induction (85.25%) and (52.75%) were produced from callus segments maintained on MS medium supplemented with 1.0 mg/l TDZ + 0.25 mg/l IBA and 1.5 mg/l BAP, respectively. The highest average shoot length was