Evaluation of antioxidant and anticancer of Cassia senna L using in vitro and in vivo methods

Abstract

Cancer is the second largest cause of death around the world. In spite of much progress in the newlinetreatment by cancer chemotherapy, there are several side effects. Hence it is Necessary to develop new newlineanticancer agents with anticancer activity but without side effects. The medicinal plants which provide newlineeffective cure and less side effects are safe, cost effective and eco friendly. Cassia species have been newlineof keen interest in pharmacological research due to their excellent medicinal values. With this newlinebackground, the present study was designed to determine antioxidants and phytochemicals of Cassia newlinesenna and to evaluate anticancer activity of C. senna using in vitro and in vivo methods. Antioxidants newlineand phytochemicals were determined in leaf and pod of C. senna. Effective extract was screened by newlinefree radical scavenging activity, chromatographical analyses and in vitro cytotoxicity assays, this was newlinefollowed by the in vivo study and characterization of active principles. The research outcome of the newlinepresent study revealed that Direct Ethanolic Extract (DEE) of C. senna leaves appear to be a good newlinesource of antioxidants and phytoconstituents. In vitro studies indicated a differential effect of DEE newlinewhich was selectively more toxic to the cancer cells and less toxic to the non-cancerous cells, which newlinemay validate DEE as a successful anticancer source. From the in vivo studies, it could be stated that newlineDEE exhibited significant antioxidant and anticancer activity in cancer induced mice. Based on the newlineresults of chromatographical and spectral analysis it can be deduced that DEE is a rich source of newlineflavonoid and alkaloids which may be the active principles responsible for the antioxidant and newlineanticancer potential of Cassia senna. newlinei) Major objectives : newlineTo determine antioxidants and phytochemicals of Cassia senna newlineTo evaluate anticancer activity of C. senna using in vitro and in vivo methods. newlineii) Methodology : newlineAntioxidants and phytochemicals were determined in leaf and pod of C. senna. newlineEffective extract was screened by free radical sc