Evaluation of Some Botanicals from JandK Possible Anticancer Role

Abstract

The present research work was carried out to examine the in vitro anticancer potential of Artocarpus heterophyllus (katahal), Cordia dichotoma (lasoda),Prunus armeniaca (apricot), the plants were collected from UT of JandK and UT of Ladakh and systematic bioassays for particular plant parts were performed on eleven human cancer cell lines (MCF-7, MDA-MB-231, N2A, SH-SY5Y, U-251, HCT-116, SW-620, A-549, MIA PaCa-2, PANC-1, PC-3) from six different tissues (breast, CNS, colon, lung, pancreatic, prostate) respectively. Methanolic extracts of all the above mentioned botanicals were prepared, used as test material. The fractions were prepared from extracts, further,the compounds were isolated from the most active fraction. The anticancer activity was determined by the cytotoxic potential of the test material. Cells were allowed to grow for 24 h on 96 well flat bottom tissue culture plates and cells were further allowed to grow in the presence of test material for 48 h. Cell growth was terminated by addition of 50% (w/v) TCA and cells were stained with SRB dye. Excess dye was removed by washing with 1% (v/v) acetic acid and bound dye was dissolved in Tris buffer and OD was taken at 540 nm. newline In the initial phase of investigation, regarding testing of extracts and fractions prepared from the above mentioned three botanicals against all the eleven human cancer cell lines. It was found that the striking observations were produced by the chloroform fraction obtained from the peel part of Artocarpus hetrophyllus as the fraction showed significant cytotoxic effect at different conc. against all the tested seven human cancer cell lines from five different origins and IC50 values were 6.40 and#956;g/mL (HCT-116), 7.04 and#956;g/mL (SH-SY5Y), 9.32 and#956;g/mL (A-549), 11.52 and#956;g/mL (MCF-7), 14.67 and#956;g/mL (MDA-MB-231), 61.27 and#956;g/mL (N2A) and 65.41 and#956;g/mL (MIA PaCa-2). In the next phase, isolation of bioactive compounds was carried out from chloroform fraction of Artocarpus hetrophyllus peel. The characterization was done by NMR (1H and13C), mass spectrometr