Biochemical Histopathological and Therapeutic Studies in Alloxan and Streptozotocin induced Diabetes Mellitus in Rabbits

Abstract

The present experimental study was designed to establish diabetes mellitus in New Zealand white rabbits using diabetogenic drugs so as to investigate/elucidate biochemical, histopathological and behavioural changes/complications. In one group of rabbits diabetes mellitus was induced by intraperitoneal administration of alloxan (at 80 mg/kg b.w.) and the other group of rabbits was made diabetic using intravenous administration of streptozotocin (at 65 mg/kg b.w.).Another group of rabbits was kept as control (normal healthy) which received normal saline. The establishment of diabetes mellitus in rabbits was confirmed by periodical elevated levels of fasting blood glucose, blood urea and serum creatinine. The subsequent effect of hyperglycemia on tissue morphology of diabetic rabbits was studied by processing of different organs viz., pancreas, kidneys, liver, lungs, heart, brain and gut of both diabetic and normal rabbits for histological/hiostopathological study using Haematoxylin and Eosin stain and modified Gomori s staining technique. newline