Studies On Design Of Novel Nanoparticulate Phytoformulations From Selected Herbs

Abstract

PART I newline6-shogaol rich ginger oleoresin extract has been standardized using a previously newlinereported method by rapid and sensitive RP-HPLC and the 6-SGL was quantified newlineat a retention time of 10.30 min at the wavelength 281 nm. The amount of 6-SGL newlinepresent in 6-SRGO was found to be 20%±2% when compare to the pure isolated newline6-SGL. The shogaols and gingerols rich 6-SRGO extract exhibited better in vitro newlineanticancer activity than pure 6-shogaol, a potent anticancer constituent of 6- newlineSRGO. Anti tumor activity in vivo reveals that 6-SRGO was effective on inhibiting newlinethe tumor progression, most likely because of synergistic activity of constituents newlinepresent in the extract. However, the exact molecular mechanism by which 6- newlineSRGO mediates its antitumor activity is to be studied. From acute toxicity studies newlineit can be concluded that the 6-SRGO is safe for internal use and can be considered newlinefor development of suitable formulation. newlineGinger is known for wide range of medicinal properties owing to its vast newlinechemical constituents including polyphenols, flavonoids and anticancer agent newlinesuch as shogaols and the gingerols. In the present study 6-SRGO was newlinestandardized using a validated High performance liquid chromatographic newlinemethod for the estimation of 6-shogaol. The standardised 6-SRGO was screened newlinefor in vitro cytotoxicity using sulforhodamine B (SRB) assay, acute toxicity and in newlinevivo anti carcinogenic effect against Dalton s Lymphoma ascites (DLA) cells. The newline6-SRGO was found to contain highest amount of shogaols and the gingerols newlinecompared to all other extracts. 6-SRGO showed better anticancer potential than newlinepure 6-shogaol on MCF-7 cell line and significant (plt0.01) in vivo newlineSUMMARY AND CONCLUSION newline newline Studies on Design of Novel Nanoparticulate Phytoformulations from Selected Herbs 113 newlineanticarcinogenic effects against DLA in comparison with 5-fluorouracil.