Extraction And Quantification Of Betalains And Antioxidants From Red Beet Beta Vulgaris By Thermal Agitation And Microwave Assisted Techniques

Abstract

newline Red beet is the commercial source of betalains that carry number of applications in the food and newlinepharmaceutical industries. The present research on betalains and antioxidants extraction from Red newlineBeet (Beta vulgaris- L.) was conducted by selecting six combinations of extraction solvent i.e.,i) newlinedistilled water ii) distilled water with 0.5% citric acid, iii) 20 % ethanol, iv) 20 % ethanol with 0.5% newlinecitric acid, v) 50 % ethanol, vi) 50 % ethanol with 0.5% citric acid under i) Thermal assisted newlineextraction (TAE- 40, 50 and 60oC) ii) Agitation assisted extraction (AAE-150, 170 190 rpm) iii) newlineMicrowave assisted extraction (MAE-160W,320W,480W for 5 minutes). The results indicate that newlineInfluence of temperature on absorption/color maxima of betacyanin (BC) and betaxanthin (BX) has newlineshown that as the temperature increased from 40 to 60oC, the extraction efficiency did increase i.e., newlinebetacyanin (BC) showed highest absorption/color maxima of 0.5224 at 60oC at 530 nm in T3 and newlinebetaxanthin (BX) also showed highest absorption/color maxima of 1.2 at 60oC at 480 nm. The newlineinfluence of agitation (150, 170 190 rpm levels) assisted extraction (AAE) on absorption/color newlinemaxima of betacyanin (BC) and betaxanthin (BX) exhibited that as agitation speed increased from newline150 to 190 rpm the extraction efficiency also increased. The AAE extraction at 190 rpm, for newlinebetacyanin (BC) exhibited highest absorption/color maxima 0.8418 at 520 nm in T4 and the lowest newlinewas 0.0034 at 545nm in T2 at 170 rpm but betaxanthin (BX) gave highest absorption/color maxima newlinei.e., 0.37 at 190 rpm at 495 nm in T2 and lowest was 0.0052 at 495 nm in T2 at 170 rpm. The influence newlineof microwave power levels indicated that at 480W betacyanin (BC) gave highest absorption/color newlinemaxima i.e., 2.283 at 530 nm in Tand#8325; and lowest was at 320W i.e. 0.0544 at 545 nm in sample Tand#8324; . The newlinebetaxanthin (BX) exhibited highest absorption/color maxima of 2.363 at 480 nm in Tand#8325; sample and newlinethe lowest was 0.0692 at 495nm in Tand#8324; at 320W