Proteomic Analysis of Seed Ageing and Seed Vigour Enhancement by Hydropriming for Abiotic Stress Tolerance in Chickpea Cicer arietinum L

Abstract

To assess the rate of seed deterioration in chickpea, seeds of variety JG-11 and newlineAnnigeri-1, were subjected to accelerated ageing conditions at 41oC and 95±5 per cent newlinerelative humidity up to 120 hours. While, for natural ageing, the seeds were packed in newlinecloth bag and stored under ambient conditions with nine per cent initial moisture. Upon newlinecomparing the germination results of accelerated and natural ageing, 48 hours of newlineaccelerated ageing (83.5 % germination) was nearly equal to 10 months of natural newlineageing (85 % germination) in case of JG-11, whereas in Annigeri-1, 48 hours of newlineaccelerated ageing (78 % germination) was nearly equal to 10 months of natural ageing newline(80 % germination). The differential protein expression of 10 months naturally aged and newline48 hours accelerated aged seeds, implicates that the process of seed deterioration under newlineaccelerated ageing is rapid than in natural ageing. Hence, the accelerated ageing test can newlinebe used to predict the relative storability of chickpea seeds. In pot experiment, the newlineinduction of physiological tolerance to drought and salinity in chickpea seedlings due to newlineseed hydropriming for 12 hours is revealed using iTRAQ based proteomic analysis. Out newlineof the 752 relatively quantified seedling proteins obtained from hydroprimed seeds newlinegrown under normal, drought and salinity stress condition, 152, 51 and 59 proteins, newlinerespectively are differentially expressed compared to unprimed control seeds. The newlinefunctional annotation of these differentially expressed proteins was grouped into six newlineclasses based on biological process of which, cellular and metabolic process group are newlinepredominant. The results from KEGG analysis disclosed that seedlings are adapted to newlinedrought and salinity mainly by up regulating proteins involved in the amino acid newlinebiosynthesis pathway, photosynthesis and by the induction of ROS scavenging newlineenzymes, chaperones, folding catalyst and ribosomal proteins for de novo protein newlinesynthesis newline