Investigating the association dissociation behaviors of amyloid like protein aggregates isolated from human cataract eye lens

Abstract

A cataract is a condition in which the main role of the eyes of living beings that is the visual functionality is hindered. The human eye lenses are mainly composed of crystallin proteins which are present as a transparent bag of sequestered proteins. These proteins are responsible for the preservation of eye lens transparency, and any modifications in their structure and composition lead to the loss of this transparency. Cataracts which are caused by age related factors are counted as the most common and primary reasons for blindness globally. Subsequently many environmental conditions along with hereditary or genetic factors also contribute to the onset of cataracts. Non hereditary age related cataracts could be considered the least understood among all types of cataracts. newlineCurrently the only method to cope with this condition is to treat it surgically as there are no such accessible preventive measures available. The surgery involves the replacement of the affected eye lens with a permanent artificial intraocular lens by surgically removing the opaque lens. These surgical interventions consist of manual small incision surgery and the procedure of phacoemulsification. As phacoemulsification is generally used in industrialized countries, small incision surgery is the most prevalent medical intervention for cataracts. These procedures bring many pre and post surgical complications with them. For many other reasons along with these there is a significant requirement to find a substitute or alternative treatment methods to prevent cataracts. It is suggested that studying the general mechanisms behind the aggregation of crystallin proteins in eye lenses will surely provide a crucial impact on the advancements of treatment methods and comprehension of cataracts. This study aims to investigate the stability of proteins acquired from the cataract eye lenses of humans. The work included the assessment of the significant factors external and internal accountable for the onset of protein aggregation that eventually leads