Detection and characterization of viruses in post transplant recipients

Abstract

newlineThe rate of undergoing renal transplantations was increase from 4 Point 9 to 5 point 4 million by 2030 Herpes viruses are most common viruses occur in post transplant period A total of 90 PRTRs were enrolled of which 51 percent n equal to 46 had CMV by real time quantitative PCR During immediate less than 3 months 56 point 7 percent n equal to 17 in late less than 3 months to 12 months 67 percent n equal to 8 very late more than 1 year post transplant 44 per cent n equal to 21 was detected. In group B n equal to 87 PRTRs symptomatic 36 percent n equal to 31 to 87 had CMV VL more than 1000 copies ml and 15 percent n equal to 13 to 87 had less than 1000 copies/ml Among asymptomatic group A 67 percent n equal to 2 had CMV VL less than 1000 copies/ml EBV was observed in 39 percent n equal to 35 of by real time quantitative PCR of which 27 percent n equal to 6 to 21 had EBV in immediate late period 41 percent n equal to 7 to 17 and very late period 43 percent n equal to 22 to 51 was observed Co occurrence of EBV and CMV was found in 21 percent n equal to 19 p0 point 05 HSV 1 was detected in 3 percent n equal to 3 during 3rd months. Seven percent n equal to 6 of VZV was detected during immediate and late post-transplant p0 point 001 A different sample whole blood urine and saliva from 30 PRTRs showed that detection rate of CMV was increased to 66 percent Three mutations in UL54 gene were observed in two PRTRs of which one novel mutation V258R TNF alpha and IL 10 was significantly higher in PRTRs n equal to 23 than in control group. Genotype 1 and genotype 2 was observed in three PRTRs of which two had mixed genotypes