Isolation and characterization of evolutionary NBS LRR genes in eggplant and watermelon

Abstract

Genetic diversity was observed in the sequences isolated, and sequences showing specific full-length conserved motifs with uninterrupted ORFs were shortlisted as eggplant and watermelon RGAs. Multiple sequence alignment of these RGAs identified the characteristic NBS-LRR motifs, and BLASTp results revealed similarity of these RGAs with other plant-pathogenesis-related proteins. Phylogeny and motif analysis revealed genetic diversity within eggplant/watermelon RGAs, and with other plant R-genes. newline