Characterization of Genotypes Based on Morphological Chemical and Molecular Markers in Scented Rice Oryza sativa L

Abstract

Locally scented popular forty one land races were collected from northern Karnataka, newlinewhich were grown over larger area, stable for specific trait(s) speciality character of aroma. newlineThese genotypes were analyzed for their seed morphometric characters by digital image newlineanalysis for the different seed parameters like length, breadth, perimeter and area for husked newlineand dehusked, and were found significantly differing to each other. newlineSignificant variations were noticed for plant morphological characters as per the newlineguidelines of PPV and FR. Some of the seed and plant morphological traits have been used newlineas key characters to identify the scented rice genotypes are leaf Auricle colour, Anthocyanin newlinepigmentation of leaf and stem, collar, ligules, stigma colour and awns. Based on these, newlinegenotypes were grouped in different types. These characters can be used for identification of newlineoff types at the time of field inspection. Genetic diversity among some of these cultivars newlineindicating that the cultivars can effectively contribute to the gene pool of aromatic rice newlinecultivars. newlineOf the 10 SSR markers used in the study only 9 markers were able to distinguish the newlinedramatic rice genotypes. A set of 4 informative SSR markers (CHR-4-8, CHR-8-34, CHR-8-29 newlineand CHR-8-6) are sufficient to analyze closely related genotypes. These primers amplified a newlinetotal of 24 profiles across 32 scented rice genotypes. Cluster analysis based on Unweighted newlinePair Group Method Average Analysis (UPGMA) led to recognition of 4 clusters. Cluster I was newlinethe largest with 26 genotypes followed by cluster III. Key characters based on aroma and newlinechemical test such as phenol, modified phenol and KOH tests are also found effective to newlinecategorise the scented rice. Both seed quality and agro-morphological characters followed by newlinemolecular marker study may be utilized to explore the relatedness and variability among the newlinescented rice lines. newline