Development and Characterization of Multifunctional Nanoparticles for Delivery of Few Anticancer Drugs and Their Evaluations

Abstract

The primary objective of this study was to enhance the therapeutic efficacy of newlinedoxorubicin (DX) and paclitaxel (PTX) medications through their integration into newlinepolyethylene glycol and polycaprolactone nanoparticles (PL NPs) and nanoparticles newlineconsisting of hyaluronic acid, polyethylene glycol, and polycaprolactone (HyAc-PEGPL newlineNPs). The investigation focused on optimizing the compositions of these NPs and newlineassessing their efficiency in delivering drugs. The NPs developed in this study were newlineemployed for encapsulating DX and PTX, and various assessment parameters were newlineevaluated, including surface morphology evaluation, DSC, XRD, entrapment newlineefficiency, in vitro drug release, hemolytic toxicity, tissue distribution study, cell newlineviability, and stability assessment. The nanoparticles exhibited a spherical structure newlineunder TEM, with HyAc-PEG-PL NPs displaying a negative zeta potential (-16.4±0.84 newlinemV), while PL NPs exhibited a negative zeta potential (-4.9±0.3 mV). The particle sizes newlinewere determined to be 268±3 nm for HyAc-PEG-PL NPs and 142±1.5 nm for PL NPs. newlineThe particle sizes were determined to be 294±12.3 nm for blank NPs and 321±08.21 newlinefor PTX- loaded HyAc-PEG-PL NPs. In terms of drug release, DX-loaded HyAc-PEGPL newlineNPs demonstrated sustained release for 96 hours, whereas DX-loaded PL NPs newlineachieved almost complete drug release within the same timeframe. Stability newlineassessments indicated that the nanoparticles remained more stable at temperatures of newline4±2°C and subsequently at 28±2°C. Hemolytic toxicity assays illustrated the high hemo newlinecompatibility of both PTX and DX loaded HyAc-PEG-PL NPs. Biodistribution analysis newlinerevealed elevated concentrations of DX and PTX in colon and tumor tissues when newlinedelivered using DX-loaded HyAc-PEG-PL NPs and PTX-loaded HyAc-PEG-PL NPs, newlineindicating enhanced targeting capabilities. DX-loaded HyAc-PEG-PL NPs also newlinexviii newlinedemonstrated superior cytotoxicity, attributed to the affinity of HyAc for overexpressed newlineCD44 receptors on HT-29 cells. For PTX, experiments involving DSC and XRD newlineindicated that PTX-HyAc-