Screening and identification of Phytopharmaceuticals for mitigation of Psoriasis vulgaris

Abstract

Psoralea corylifolia(Leguminosae) is an annual herb, found throughout India, commonly known as Bakuchi. The whole plant, especially its seed or fruit, has significant medicinal properties for the treatment of many diseases. The plant contains coumarins, meroterpenes, flavonoids, sterols andvolatile oil. Berberisaristatais an important multipurpose shrub native to hilly parts of Northern India, belonging to the Berberidaceae family and commonly known as Daruhaldi. It is reported tocontain isoquinoline alkaloids, flavonoids and resins. Both the plants were evaluated for pharmacognostic study which includes macro and microscopic evaluation, determination of physicochemical parameters in a systematic way. New isolation have been developed for the active principles present in P. corylifolia using flash chromatography technique. A simple, validated HPTLC and UHPLC PDA with ESI MS-MS methods were developed to estimate the major bioactives from both the plant. In-vitro studies using THP-1 cell lines using MTT assay was conducted. Preclinical for safety and IMQ (imiquimod) induced psoriatic lesion model was studied for Psoralea corylifolia and Berberis aristata using different extracts, combination of extracts and bioactives. The characteristic features for morphological, microscopical and physico-chemical parameters that are essentially required for the quality evaluation of both the plants are recorded systematically. Psoralen, bakuchiol, 5-hydroxy xanthotoxin and methyl 4-hydroxy benzoate were isolated by column chromatography and purified by flash chromatography. Validated and Simultaneous HPTLC methods designed for the quantitative and qualitative analysis of the markers in both the medicinal plants revealed the presence of bakuchiol (1.20 %w/w), psoralen (0.585 %w/w) and bavachin (0.285 %w/w) in P. corylifolia, and berberine (1.17 % w/w), palmatine(0.84 % w/w), berbamine (0.77 %w/w), jatrorrhizine (0.12 % w/w) and magnoflorine (0.10 % w/w) in B. aristata. Additionally, UHPLC MS/MS methods developed and validated sep