Efficient Routing and Synthesis on MEDA Based and conventional Digital Microfluidic Biochip

Abstract

Digital Microfluidic Biochips (DMFBs) are widely used in pharmaceutical and medical research fields, particularly for point-of-care diagnosis, analysing toxicity, sequencing of DNA, and many other biochemical investigations. DMFB is maneuverer to facilitate various operations like sample preparation, mixing with other reagents, and splitting micro-volume droplets. Traditional DMFBs have many drawbacks, including certain fluidic limitations, an internal configuration of the electrodes, and different volumes of the droplets. Due to some fluidic limitations and internal chip-electrode architecture, it is difficult to manage the sizes and volumes of the different droplets. The capabilities of integrated sensors for in-the-moment observation are also constrained. Conventional DMFBs are frequently unable to correct mistakes made throughout an experiment, which results in unfavourable outcomes. Compared to conventional DMFB, recent developments in Paper-Based DMFB (PBDMFB) open new possibilities for affordable diagnostic procedures and medical care. Testing process and fault diagnosis have been performed on a PB-DMFB due to their distinct physical features, which differ from typical DMFBs or Lab-on-a-Chip (LoC)s. The paper-based microfluidic arrays are now used to carry out basic fluidic activities such as droplet routing, mixing, splitting, incubation, and detection. However, functional testing of such chips can guarantee the dependability of those fundamental fluidic functions. Routing on such chips is subject to several restrictions with conventional DMFBs. The Thesis presents different kinds of defects on a Paper Based Digital Microfluidic Biochip. There have been novel diagnosis models based on routing that take into account various single-site and multiple-site defects along the routing path. In order to complete 100% bioassay synthesis on a PB-DMFB, the proposed approach has taken into account single and dual-site defects in various mixing modules on PB-DMFB and offered strategies for addressing such mixing