Isolation and characterization of the components from Amaranthus species and study of Bioactivity

Abstract

Isolation and Characterization of the Components from Amaranthus Species and Study of bioactivity newlineThe work is described in the present thesis covers the study of Amaranthus tricolor Linn, Amaranthus viridis Linn plants of Amaranthaceae family. The thesis is divided into total six chapters. Thesis includes proximate and phytochemical analysis, bioactive study of extracts and isolated compounds. Isolation, characterization and structure determination of compounds obtained is performed with the help of modern spectral techniques. newlineChapter 1 includes the introduction A. tricolor and A. viridis, along with the techniques used and biological activities employed. Chapter 2 provides detailed literature review of both leafy vegetables, depicting their nutraceutical, therapeutic and pharmaceutical importance. The previously reported work regarding the phytochemical constitution and their bioactivity are described. newlineChapter 3 defines the aims and objectives carried out during the present research work. Chapter 4 contains the materials and methods used to perform the various experiments. Standard methods are employed for proximate and phytochemical studies. Different chromatographic techniques are acquired to isolate pure compounds. Detailed information is mentioned for the assessment of the isolated four molecules and various biological activities examined are mentioned. newlineChapter 5 describes the result and discussion about the isolation, characterization and structure determination of compounds obtained is performed with the help of modern spectral techniques such as FT-IR, UV, PMR, CMR, DEPT-135°, COSY, NOSEY, HMBC, HSQC, HRMS and MS-TOF. Chapter 6 consists of summary and conclusion. Four novel compounds are isolated. Compound 1 (C40H82), long straight chain hydrocarbon, obtained from steam volatile matter of A. viridis. Compound 2 (C41H70O3Cl2) is a steroidal compound. Compound 3 (C48H92O3Cl2) is an open chain organic compound. Compound 4 (C47H80O15) is a carbohydrate like organic compound.