comparison of the tolerability and effectiveness of amikacin administered through autoinjector and manual injection using biochemical and histological parameters in rats and rabbits

Abstract

Aim newline The aim of the present study is to develop an antibacterial autoinjector newlinefor emergency management for infection control. newline2.2 Objectives newlineThe objectives of the present study are newlinei) To compare the biochemical parameters in Wistar rats newline administered with amikacin by autoinjector and with manual newline injection. newlineii) To compare the oxidative stress parameters and histological newline alterations in the liver and kidney of Wistar rats, administered newline with amikacin by autoinjector and with manual injection newlineiii) To find out the effectiveness of amikacin administered through newline autoinjector and manual injection in infected excision wound newline model of Wistar rats. newlineiv) To compare the haematological and biochemical parameters in newline New Zealand white rabbits, administered with amikacin by newline autoinjector and manual injection. newline newline2.3 Ethical Considerations newline The experimental protocols of the present studies have been approved newlineby the Institutional Animal Ethical Committee, Saveetha University.The studies newlinehave been done as per the guidelines of the Committee for the Purpose of newlineControl and Supervision of Experiments (CPCSEA), New Delhi, India.The newlineapproval numbers were SU/BRULAC/RD/001/2013 dated 11th July 2013 for newlinerabbit study and SU/BRULAC/RD/010/2014 dated 30th May 2014 for rat newlinestudies. Rat and rabbit models were chosen for the preclinical studies as they newlineare very docile in nature and easy to handle. The amikacin cartridges used for newlinethe study were stored safely with utmost care and in aseptic conditions newlinethoughout the stud newline