Population dynamics and in silico approaches of larvicidal activity of selected plant extracts against filarial vector culex quinquefasciatus diptera culicidae

Abstract

Population study of mosquito species was undertaken in four areas of Coimbatore namely newlineAvinashilingam university campus Race course Sennanur and Kavundampalayam Fort newlinenightly collections of adult mosquitoes were made from July 2010 to June 2012 During the newlinestudy period meteorological parameters such as temperature relative humidity and rainfall newlinewere recorded to assess their influence on the mosquito population The result indicated that C newlinequinquefasciatus was the predominant species in selected four areas followed by Armigerous newlinesubalbatus and Aedes aegypti The efficacy of petroleum ether chloroform and ethanol extract newlineof leaf pericarp and seed of Capsicum annuum L and Momordica charantia L were tested on newlinethe fourth instar larvae of C quinquefasciatus at varying concentrations Among the two plant newlineextract chloroform extract of seed of C annuum L produced 99 point nine percentage mortality at 48hr in 400ppm Preliminary screening of the extract and identification was done by colour tests The newlineGC MS analysis of the selected plants extracts were carried out Modelled 3 D structure of newlinesalivary gland protein of C quinquefasciatus was validated using PROCHECK and ERRAT newlineThe total energy values of the predicted 3D model were calculated as good quality of most newlinefavoured region in Ramachandran plot 13 compounds were selected for docking studies out of newlinewhich 10 compounds binded with odorant binding protein of C quinquefasciatus and newlinecarthamine showed a good glide score followed by Gallic acid Eight out of 13 compounds newlinebinded with salivary gland protein of C quinquefasciatus and gallic acid showed a good glide newlinescore followed by 2 5 dihydroxy benzoic acid Therefore naturally existing plant based newlinepesticides were efficient to control mosquitoes to protect environment newline