Extraction Isolation and Characterization of Alstonia scholaris and Caesalpinia crista and Screening Their Pharmacological Activity

Abstract

Due to their versatile applications, plant derived substances are gaining great interest and newlinethere has been an increased focus on plant research all over the world. Also traditional plant newlinederived drugs promise safety as compared to the synthetic ones that are considered as newlinedangerous to human and environment. newlineThe present research work aimed to evaluate the phytochemical and pharmacological profile newlineof ethanolic extracts of stem bark of Alstonia scholaris and seeds of Caesalpinia crista. newlinePreliminary phytochemical screening of the ethanolic extracts was performed through various newlinechemical tests to identify the bioactive phytoconstituents like flavonoids, alkaloids, tannins, newlinesaponins, phenolics, triterpenoids, carbohydrates, etc. TLC also revealed the presence of newlinevarious phytoconstituents in the prepared extracts. Various secondary bioactive metabolites newlinelike phenolics and flavonoids were also estimated quantitatively. newlineFive compounds were isolated by subjecting the ethanolic extract of selected plant materials newlineto column chromatography technique. All the isolated compounds were characterized through newlineIR, mass and 1H and 13C NMR spectroscopy. newlineThe pharmacological evaluation comprised of assessing antioxidant and hepatoprotective newlineefficacy of ethanolic extract of stem bark of Alstonia scholaris and seeds of Caesalpinia newlinecrista. newlineThe in vitro antioxidant potential was assessed by DPPH free radical scavenging method. The newlinein vivo hepatoprotective activity was evaluated against carbon tetrachloride induced newlinehepatotoxicity in Wistar rats. In both the cases, i.e. antioxidant and hepatoprotective newlineactivities, the ethanolic extract of stem bark of Alstonia scholaris (EEAS) and seeds of newlineCaesalpinia crista (EECC) were found active. The research affirms a future study involving newlinethe isolated compounds for screening their detail biological activity so that they can be used newlineas new therapeutic moiety or as lead compounds for future research. newline