Formulation Development and Evaluation of Resveratrol Loaded Microcarrier System for the Managemnet of Ulcerative Colitis

Abstract

The present work deals with the formulation and optimization of pectin-based colon newlinespecific microparticles and pellets containing Resveratrol. Pectin based microparticles newlineand pellets coated with Cellulose acetate phthalate containing Resveratrol were newlineformulated with in five different batches (MF1-MF5 and PF1-PF5). Box-Behnken design newline(BBD) matrix was applied to optimise the core formulations. Systematic optimization of newlineuncoated microsphere was done by Box-Behnken design (BBD) employing three critical newlinefactors, pectin concentration (A); Stirring speed (B) and Span 80 concentration (C). newlineParticle size (and#956;m), entrapment efficiency (EE %) and recovery (%) were the key CQAs. newlinePectin microparticles and pellets were coated by CAP using the oil-in-oil solvent newlineevaporation method. Characterisation of the formulations were done by determination of newlinepercentage yield, micromeritics properties, drug loading and entrapment efficiency newlinefollowed by particle size analysis morphological analysis using transmission electron newlinemicroscopy (TEM) scanning electron microscopy (SEM). Drug-excipients compatibility newlinestudy was also performed by FTIR spectrometric analysis. The formulations were newlineevaluated by in vitro dissolution test in simulated gastric, intestinal and colonic fluid with newlineand without 4% w/v rat caecal matter. The formulations were evaluated by in vitro newlinedissolution test in simulated gastric, intestinal and colonic fluid with and without 4% w/v newlinerat caecal matter. newline