Development Characterization and In vivo Assessment of Insulin Loaded Thermosensitive in situ Nanogel for Nasal Delivery

Abstract

newline The mucoadhesive gel formulations are helpful to prolong the residence time at the nasal absorption newlinesite and thereby facilitate the uptake of drug. The objective of the present study was to develop a newlinethermosensitive in-situ nanogel system based on chitosan and tri-polyphosphate for nasal delivery newlineof insulin. Nanogel containing insulin was prepared through an ionic gelation method. The newlineconcentration of the components was optimized during formulation development and then newlinecharacterized in terms of drug content, mucoadhesive strength, pH, spreadability and stability study newlineand drug release behavior. The drug release results were fitted on five mathematical models to newlinechoose the model best describing the phenomenon. The in-vitro release of insulin from gel network newlinewas observed spectrophotometrically which was good enough to maintain blood glucose level for newline14 hours. Then the nano-formulation and insulin SC injection as control were administered in the newlinenasal cavity for rats and after 2, 4, 6, 8 and 10 hrs their blood glucose levels, serum insulin level newlineanalyzed for antidiabetic activity. The observed in-vitro and in-vivo results indicate that the newlineproposed thermosensitive in-situ gelling system has substantial potential as nasal delivery system newlinefor insulin. newlineKEYWORDS: Mucoadhesive, insulin, in Situ Nano gel, ionic gelation method, antidiabetic newlineactivity