Morphological and functional characterization of boar spermatozoa on incubation in capacitating media and preservation

Abstract

A total of 24 ejaculates comprising 6 ejaculates from each of four HD-K75 boars newlineof 10-12 months age maintained at ICAR - All India Coordinated Research Project newline(AICRP) on Pig C.V.Sc, A.A.U., Khanapara, Guwahati are being selected for the present newlinestudy. The semen was collected by simple fist method twice weekly to study the newlinemorphological and functional characterization of in-vitro capacitated and preserved boar newlinespermatozoa. After initial evaluation (volume, concentration and initial motility), the fresh newlinesemen was split into three parts. One part of the semen was used for fresh semen newlineevaluation, second for capacitation and the other for preservation. For capacitation, the newlinesemen was incubated in TALP and m-KRB media at 37oC for 5 hours. For preservation newlinesemen was extended (1:4) in BTS and GEPS extenders and held at 22ºC for 4 hours. The newlineextended semen was then preserved at 15oC in BOD incubator upto 120 hours. newlineThe overall mean of strained volume of semen, initial motility, hyperactivated newlinespermatozoa, sperm concentration, live spermatozoa, live acrosome reacted spermatozoa newlineand per cent hypo-osmotic swelling test (HOST) was 220.65±5.34 ml, 83.29±0.92 per newlinecent, 92.21±0.54 per cent, 270.87±2.94 million per ml, 90.82±0.83 per cent , 82.76±0.36 newlineper cent and 65.06±0.27 per cent and the overall range being 150-265 ml, 78 to 95 per newlinecent, 88 to 95 per cent, 245- 298 million per ml, 86 to 95, 79-86 and 62 to 78 per cent newlinerespectively. newlineSperms were suspended in TALP media and m-KRB media and incubated for 5 newlinehours at 370C for in-vitro capacitation and evaluation was carried out at 0, 3 and 5 hours of newlineincubation. In the present study, the highest hyperactivated motility was observed at 3 newlinehours of incubation, from 18.51% at 0 hour to 57.32% in TALP and 17.96% at 0 hour to newline43.25% at 3 hour in m-KRB, the hyperactivated motility of spermatozoa increased newlinesignificantly upto 3 hours then it decreased upto 44.72 in TALP and 43.25 at 5 hours of newlineincubation. The overall mean live acrosome reacted spermatozoa per cent declined from newline85.31% to 3