Cloning expression purification structure and functional characterization of rhamnogalacturonan lyase ctrgl from family 11 polysaccharide lyase pl11 and associated carbohydrate binding module 35 cbm35 from clostridium thermocellum atcc 27405

Abstract

Pectin is an important component of plant cell wall and is composed of Rhamnogalacturonan I RG I Rhamogalacturonan II and Homogalacturonan Pectin degrading enzymes such as rhamnogalacturonan lyase are industrially important Full length rhamnogalacturonan lyase CtRGLf from Clostridium thermocellum and its truncated derivatives CtRGL catalytic module Rgl CBM35 carbohydrate binding module were cloned expressed functionally and structurally characterized CtRGLf and CtRGLwere found to newline newline